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Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Quality Control01:05

Quality Control

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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
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Nucleic Acid Structure01:25

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
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Electricity-Free, Sequential Nucleic Acid and Protein Isolation
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Nucleic Acid Isolation and Quality Control.

Ling Dong1, Janice Yoshizawa1, Xinmin Li2

  • 1Technology Center for Genomics and Bioinformatics (TCGB), Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
PubMed
Summary
This summary is machine-generated.

This chapter details practical manual and automated methods for isolating genomic DNA and RNA. It also covers quality control for DNA, RNA, and next-generation sequencing libraries.

Keywords:
Genomic DNA isolationGenomic DNA quality controlNext generation sequencing library quality controlRNA isolationRNA quality control

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Accurate nucleic acid isolation is fundamental for downstream molecular analyses.
  • Standardized quality control is essential for reliable experimental outcomes.

Purpose of the Study:

  • To present practical manual and automated methods for genomic DNA and RNA isolation.
  • To summarize current quality control techniques for nucleic acids and sequencing libraries.

Main Methods:

  • Description of established manual DNA/RNA extraction protocols.
  • Overview of automated high-throughput nucleic acid isolation systems.
  • Review of quality control assays for DNA/RNA integrity and purity.
  • Summary of quality control for next-generation sequencing library preparation.

Main Results:

  • Identification of efficient and scalable nucleic acid isolation techniques.
  • Compilation of widely-used quality control methods for genomic samples.
  • Assessment of methods suitable for various biological sources.

Conclusions:

  • The chapter provides a comprehensive guide to practical nucleic acid isolation and quality control.
  • It serves as a resource for researchers seeking robust methods in molecular biology and genomics.