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Updated: Jan 31, 2026
12:15
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
11.6K
Humanization and Simultaneous Optimization of Monoclonal Antibody.
Taichi Kuramochi1, Tomoyuki Igawa2, Hiroyuki Tsunoda3
1Chugai Pharmabody Research Pte. Ltd., Singapore, Singapore. kuramochitic@chugai-pharm.co.jp.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
Summary
Antibody humanization reduces immunogenicity risk for therapeutic antibodies. Optimizing multiple properties simultaneously accelerates the development of qualified clinical candidates.
Area of Science:
- Biotechnology
- Immunology
- Pharmaceutical Science
Background:
- Antibody humanization is crucial for minimizing immunogenicity of animal-derived antibodies, with widespread application in marketed therapeutics.
- Developing therapeutic antibodies requires consideration of multiple properties beyond humanization.
Purpose of the Study:
- To outline critical properties for therapeutic antibody development.
- To describe methodologies for antibody engineering and optimization.
Main Methods:
- Review of essential antibody properties: immunogenicity, binding affinity, physicochemical stability, host cell expression, and pharmacokinetics.
- Discussion of antibody engineering techniques for simultaneous optimization.
Main Results:
- Simultaneous optimization of multiple antibody properties is key.
- This integrated approach can shorten research and development timelines.
Conclusions:
- Optimizing antibody properties alongside humanization is essential for efficient drug development.
- Accelerating the identification of clinical candidates can speed up clinical trials.
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