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Updated: Feb 1, 2026
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood
Published on: January 9, 2018
Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry
Satoshi Yamashita1, Nozomu Tawara2
1Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan. y-stsh@kumamoto-u.ac.jp.
Abstract:
Sporadic inclusion body myositis (sIBM) is a chronic and progressive inflammatory myopathy that is the commonest among population over 50s. Recently, autoantibodies against cytosolic 5'-nucleotidase 1A (cN1A) have been identified in plasma and serum samples from patients with sIBM. So far, various methods have been established to detect the anti-cN1A autoantibodies, which showed a clinical utility of detection of the autoantibodies in the diagnosis of sIBM. Here we describe a novel cell-based assay for detection of the autoantibodies, which seems equivalent in sensitivity and better in specificity for the diagnosis of sIBM, compared with the previous methods.
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