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Screening of Streptococcus Suis serotype 2 resistance genes with GWAS and transcriptomic microarray analysis
Zhe Ma1,2,3, Haodan Zhu4, Yiqi Su1,2
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
BMC Genomics
|December 14, 2018
Summary
Researchers identified two key genes, Ubac1 and Epn 1, associated with Streptococcus Suis serotype 2 (SS2) resistance in mice. This discovery aids in developing disease resistance breeding strategies for swine.
Area of Science:
- Genomics
- Immunology
- Animal Science
Background:
- Swine streptococcosis, caused by Streptococcus Suis serotype 2 (SS2), results in significant economic losses.
- Disease resistance breeding is crucial for mitigating SS2 impact in swine populations.
- Genome-Wide Association Studies (GWAS) and transcriptomic microarray technologies offer powerful tools for identifying SS2 resistance genes.
Purpose of the Study:
- To identify genes associated with Streptococcus Suis serotype 2 (SS2) resistance using a combined GWAS and gene expression approach.
- To investigate the genetic basis of SS2 resistance in mice as a model for swine.
Main Methods:
- Generation of an F2 mouse population from SS2-resistant (C57BL/6) and SS2-susceptible (A/J) strains.
- Genome-Wide Association Study (GWAS) analysis to identify significant single nucleotide polymorphism (SNP) sites.
- Transcriptomic microarray analysis to compare gene expression profiles under SS2 infection pressure.
Main Results:
- GWAS identified 286 significant SNPs associated with SS2 resistance.
- Microarray analysis revealed 251 differentially expressed genes between resistant and susceptible mouse strains during SS2 infection.
- Two genes, UBA domain containing 1 (Ubac1) and Epsin 1 (Epn 1), were significantly associated with SS2 resistance after integrating GWAS and gene expression data.
- Gene Ontology analysis highlighted nine immune-function-related genes potentially involved in SS2 resistance.
Conclusions:
- This study pioneers the use of both SNP chip and gene expression profile chip for SS2 resistance gene identification in mice.
- The identified genes, Ubac1 and Epn 1, provide valuable targets for enhancing SS2 resistance in swine through breeding programs.
Keywords:
Genome-wide association studyResistance genesStreptococcus suis serotype 2Transcriptome analysisMore Related Videos
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