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Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
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Baculovirus as an efficient vector for gene delivery into mosquitoes
Nenavath Gopal Naik1, Yu-Wen Lo1, Tzong-Yuan Wu2,3
1Institute of Molecular Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, 115, Taiwan, Republic of China.
Scientific Reports
|December 14, 2018
Summary
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) offers efficient gene delivery in mosquito cells and insects. This baculovirus vector enables high gene expression for studying mosquito biology and arboviruses without harming cells or tissues.
Area of Science:
- Molecular Biology
- Entomology
- Virology
Background:
- Efficient gene delivery is crucial for research on mosquito biology and arboviruses.
- Current methods for mosquito gene delivery are limited, hindering research progress.
Purpose of the Study:
- To evaluate Autographa californica multiple nucleopolyhedrovirus (AcMNPV) as a gene delivery vector in mosquito cells and organisms.
- To establish a flexible and efficient gene delivery system for mosquito research.
Main Methods:
- Transduction of mosquito cell lines with AcMNPV.
- Assessment of gene expression levels and cell viability post-transduction.
- In vivo transduction of mosquito larvae and adults.
- Expression of influenza virus neuraminidase (NA) in mosquitoes.
Main Results:
- AcMNPV efficiently transduced various mosquito cell types, surpassing mammalian cell lines and plasmid transfection.
- High gene expression was achieved without negative effects on cell propagation or viability.
- AcMNPV successfully transduced mosquito larvae and adults across different genera, with minimal tissue barriers.
- Demonstrated successful expression of a foreign gene (influenza NA) in mosquitoes.
Conclusions:
- Baculovirus (AcMNPV) serves as a powerful and versatile gene delivery vector for both in vitro and in vivo applications in mosquitoes.
- This system significantly advances research in mosquito gene regulation, development, and the study of mosquito-borne diseases.
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