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Updated: Feb 1, 2026

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
Published on: April 27, 2018
CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids
Else Driehuis1,2, Hans Clevers1,3
1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), The Netherlands.
Abstract:
TMPRSS2-ERG fusions are common genetic events in prostate cancer. Until now, this genetic alteration was modelled by ERG overexpression. In this short communication, we report the creation of mouse prostate organoids that have undergone gene fusion through a CRISPR/Cas9-based strategy. The genetic fusion of TMPRSS2 and ERG results in ERG overexpression. This effect is androgen receptor-mediated, as expression of the fusion transcript can be restored to wildtype ERG levels by treatment with the androgen receptor antagonist Nilutamide.
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