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Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization.

Huanhuan Joyce Chen1,2, Michael L Shuler3

  • 1Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 14, 2018
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Summary
This summary is machine-generated.

Researchers engineered a bioartificial colon using decellularization and recellularization. This novel tissue engineering method regenerates human intestinal tissue and aids in identifying cancer driver genes.

Keywords:
DecellularizationDisease modelingHuman intestineRecellularizationTissue engineering

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Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Gastroenterology

Background:

  • Decellularization and recellularization are established tissue engineering techniques.
  • Existing protocols for decellularization/recellularization vary significantly across different organs and tissues.
  • There is a need for standardized and effective methods for engineering complex organs like the colon.

Purpose of the Study:

  • To develop and describe an effective method for engineering a bioartificial colon.
  • To demonstrate the successful decellularization of human intestinal tissue.
  • To show the successful recellularization of the acellular matrix with cell cultures for intestinal regeneration.

Main Methods:

  • Human intestinal tissues were subjected to a complete decellularization process to remove all native cells.
  • The resulting acellular tissue matrix was repopulated with specific cell cultures.
  • The engineered construct was assessed for its potential as a bioartificial colon.

Main Results:

  • A novel method for engineering a bioartificial colon from human intestinal tissue was successfully developed.
  • Complete removal of original cells from the intestinal tissue was achieved.
  • Repopulation of the acellular matrix with cell cultures demonstrated the viability of the tissue engineering approach.

Conclusions:

  • This study presents a novel and effective tissue engineering strategy for human intestinal regeneration.
  • The developed bioartificial colon serves as a promising platform for studying intestinal biology.
  • The engineered tissue holds potential for identifying critical cancer driver genes in the human intestine.