Development of Virus Resistance Transgenic Cotton Using Cotton Leaf Curl Virus Antisense ßC1 Gene

Cotton (Gossypium hirsutum L.) is the most economically important crop in the world and produced 90% of the total natural cellulose fiber which is utilized to make cotton fabrics. The production of cotton is affected by many several diseases, and among them, viral disease, especially leaf curl, is the most destructive disease caused by a begomovirus transmitted by whiteflies vector. Plant biotechnology has provided an opportunity to develop transgenic plant with variable traits against biotic and abiotic stress such as resistance against pathogens, yield, quality, and salinity. Transgenic cotton (Gossypium hirsutum L., cv. Coker 312) plants were raised against leaf curl disease using bC1 gene in antisense orientation through Agrobacterium-mediated transformation somatic embryogenesis system. In this chapter, a standardized protocol will be given to raise virus resistance transgenic cotton.