Related Experiment Video

Updated: Jul 12, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 18, 2010

[Effect of inhibitors and cations on the proteolytic activity of Bacillus mesentericus]

A A Imshenetskiĭ, N G Nesterova, G V Cherkesova

    Mikrobiologiia
    |May 1, 1988
    PubMed

    Insights

    Proteolytic enzymes from two Bacillus mesentericus strains were studied. Strain 64

    Area of Science:

    • Microbiology
    • Enzymology

    Background:

    • Bacillus mesentericus produces proteolytic enzymes.
    • Understanding enzyme activity is crucial for industrial and therapeutic applications.

    Purpose of the Study:

    • To comparatively analyze the effects of inhibitors and metal cations on two Bacillus mesentericus proteinases.
    • To determine if the proteinases from strain 8 and strain 64 M-variant are distinct.

    Main Methods:

    • Comparative study of proteolytic activity.
    • Investigation of bivalent metal cations (Mn2+, Ca2+, Fe2+, Zn2+, Mg2+, Co2+, Cu2+) effects.
    • Enzyme characterization as serine proteinases.

    Main Results:

    • Both enzymes identified as serine proteinases.
    • Proteinase from strain 64 demonstrated metal-dependent activity.
    • Ca2+ and Mg2+ stimulated strain 64 proteinase; Fe2+ and Zn2+ inhibited it.
    • Metal ions had minimal effect on strain 8 proteinase.

    Conclusions:

    • The two Bacillus mesentericus proteinases exhibit distinct properties.
    • Strain 64 proteinase is a metal-dependent serine proteinase, unlike strain 8 proteinase.

    More Related Videos

    A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice07:54

    A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

    Published on: July 25, 2017

    Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

    Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

    Published on: October 3, 2018

    Related Concept Videos

    Enzyme Inhibition01:30

    Enzyme Inhibition

    Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
    Antimicrobial Proteins01:23

    Antimicrobial Proteins

    Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
    Interferons
    Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
    Inhibitors of Gram-positive Cell Wall Synthesis01:23

    Inhibitors of Gram-positive Cell Wall Synthesis

    Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
    Inhibitors of Bacterial Protein Synthesis01:25

    Inhibitors of Bacterial Protein Synthesis

    Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...