Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Toxic Reactions: Overview01:26

Toxic Reactions: Overview

2.0K
When toxic substances penetrate the human body, they disseminate to various tissues, undergoing metabolic changes. This process yields reactive metabolites that may covalently bind with specific target molecules, resulting in toxicity.
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
2.0K
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

609
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
609
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

234
Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
234
Assessment of blood pressure in brachial artery(one-step method)01:15

Assessment of blood pressure in brachial artery(one-step method)

1.2K
This procedural guide systematically measures blood pressure using an oscillometric digital sphygmomanometer, emphasizing accuracy, patient safety, and comfort.
Prepare for the Procedure:
1.2K
Assessment of blood pressure in brachial artery(two-step method)01:23

Assessment of blood pressure in brachial artery(two-step method)

1.6K
Measuring blood pressure is a fundamental skill in healthcare that aids in diagnosing and monitoring hypertension and other cardiovascular conditions. An aneroid sphygmomanometer, commonly used in clinical settings, offers a manual and precise method for blood pressure measurement. The technique for using this instrument involves specific steps that must be carefully executed to ensure accuracy. The following detailed description outlines a two-step technique for assessing blood pressure using...
1.6K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

16.3K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physicochemical Characterization of Hyaluronic Acid-Methylcellulose Semi-Gels for Mitochondria Transplantation.

Journal of biomedical materials research. Part B, Applied biomaterials·2025
Same author

DNA Packaging and Polycation Length Determine DNA Susceptibility to Free Radical Damage in Condensed DNA.

The journal of physical chemistry. B·2024
Same author

Development of Poly(acrylamide)-Based Hydrogel Composites with Powdered Activated Carbon for Controlled Sorption of PFOA and PFOS in Aqueous Systems.

Polymers·2023
Same author

Demonstration of Hollow Fiber Membrane-Based Enclosed Space Air Remediation for Capture of an Aerosolized Synthetic SARS-CoV-2 Mimic and Pseudovirus Particles.

ACS ES&T engineering·2023
Same author

The Impact of Solution Ionic Strength, Hardness, and pH on the Sorption Efficiency of Polychlorinated Biphenyls in Magnetic Nanocomposite Microparticle (MNM) Gels.

Gels (Basel, Switzerland)·2023
Same author

Magnetic Nanocomposites for the Remote Activation of Sulfate Radicals for the Removal of Rhodamine B.

Nanomaterials (Basel, Switzerland)·2023
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 1, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
09:49

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

24.7K

In Vitro Methods for Assessing Nanoparticle Toxicity.

Dustin T Savage1, J Zach Hilt1, Thomas D Dziubla2

  • 1Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2018
PubMed
Summary
This summary is machine-generated.

Nanoparticle production increases public health risks. Their properties enhance toxicity, necessitating advanced assays to predict harm from these nanomaterials.

Keywords:
BiocompatibilityIn vitro assaysMechanisms of toxicityNanomaterialsNanoparticle characterizationNanoparticle toxicityNanoparticle-cell interactionReactive oxygen species

More Related Videos

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

5.3K
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
05:27

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

Published on: December 25, 2016

15.6K

Related Experiment Videos

Last Updated: Feb 1, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
09:49

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

24.7K
Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

5.3K
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
05:27

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

Published on: December 25, 2016

15.6K

Area of Science:

  • Environmental Science
  • Toxicology
  • Nanotechnology

Background:

  • Increasing nanoparticle production and environmental release pose public health concerns.
  • Nanoparticle physicochemical properties contribute to their toxicity.
  • Understanding nanoparticle uptake and biological effects is crucial.

Purpose of the Study:

  • To highlight the public health risks associated with nanoparticles.
  • To emphasize the need for characterizing nanoparticle properties for toxicity prediction.
  • To review current assay methods for detecting nanoparticle-induced cellular damage.

Main Methods:

  • Review of nanoparticle properties (size, morphology, charge, coating, composition).
  • Analysis of various assay methods (endotoxin, LDH, apoptosis, oxidative stress).
  • Spectroscopic investigation of cellular uptake and barrier permeation.

Main Results:

  • Nanoparticle properties significantly influence biological responses and toxicity.
  • Assays can detect cellular damage biomarkers.
  • Nanoparticles can penetrate biological barriers and reach cytotoxic locations.
  • Lack of deterministic trends requires system-specific evaluation.

Conclusions:

  • Comprehensive characterization and assay combination are vital for new nanoparticle systems.
  • Nanoparticle interactions with proteins and substrates challenge traditional assays.
  • Future work should focus on modifying assays for accurate nanoparticle toxicity evaluation.