Measurement of Nanoparticle-Induced Mitochondrial Membrane Potential Alterations

Mitochondria hold a critical role in cell metabolism and homeostasis. Mitochondrial injury plays central part in deciding cell fate especially in programmed cell death pathways. Various nanomaterials lead to different cell death modalities by inducing mitochondrial injury. Mitochondrial injury is manifested as multiple biochemical events ranging from altered energy production, mitochondrial outer membrane permeability, release of pro-apoptotic BCl-2 family proteins, loss of mitochondrial inner membrane potential, mitochondrial swelling, and disruption of mitochondrial structure leading to eventual lysis of mitochondria. Mitochondrial membrane permeability (loss of mitochondrial membrane potential) is a critical event in deciding cell fate. This chapter presents an overview of nanomaterial-induced loss of mitochondrial membrane potential and discusses potential nano-specific artifacts in these assays. Finally, a detailed methodology to accurately quantify and validate the loss of mitochondrial membrane potential after nanomaterial exposures is presented.