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Microbacterium suaedae sp. nov., isolated from Suaeda aralocaspica
Zhi-Nan Zhu1, Yan-Ru Li1, Yu-Qian Li2
11Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Ürümqi 830054, PR China.
International Journal of Systematic and Evolutionary Microbiology
|December 15, 2018
Summary
Two novel bacterial strains were discovered in a Chinese desert, belonging to the Microbacterium genus. These extremophilic bacteria, named Microbacterium suaedae, thrive in harsh desert conditions and expand our understanding of microbial diversity.
Area of Science:
- Microbiology
- Bacteriology
- Extremophile Research
Background:
- The Gurbantunggut desert harbors unique microbial life adapted to extreme environments.
- Halophytes, like Suaeda aralocaspica, are potential sources of novel microorganisms.
- Understanding bacterial diversity in arid regions is crucial for ecological studies.
Purpose of the Study:
- To isolate and characterize novel bacterial strains from the halophyte Suaeda aralocaspica.
- To determine the phylogenetic and taxonomic position of the isolated strains.
- To propose a new species within the genus Microbacterium.
Main Methods:
- Isolation of bacterial strains from Suaeda aralocaspica.
- Gram staining, aerobic cultivation, and growth condition analysis (temperature, pH, NaCl).
- Phylogenetic analysis using 16S rRNA gene sequences.
- Genomic DNA G+C content determination.
- Analysis of cell-wall amino acids, whole-cell sugars, peptidoglycan acyl type, fatty acids, menaquinones, and polar lipids.
Main Results:
- Two Gram-positive, aerobic, non-motile, short rod strains (YZYP 306T and YZGP 509) were isolated.
- The strains exhibited tolerance to a wide range of temperatures (4-40/42 °C), pH (6.0-12.0), and NaCl concentrations (0-15%).
- Phylogenetic analysis based on 16S rRNA gene sequences indicated the strains belong to the genus Microbacterium, with highest similarity to Microbacterium indicum BBH6T (97.8%).
- Average nucleotide identity (ANI) between YZYP 306T and M. indicum BBH6T was 78.3%.
- Genomic DNA G+C content was approximately 68.5%.
- Chemotaxonomic analyses (cell-wall amino acid, sugars, fatty acids, etc.) supported the classification within the genus Microbacterium.
Conclusions:
- The isolated strains, YZYP 306T and YZGP 509, represent a novel species within the genus Microbacterium.
- The proposed name for the novel species is Microbacterium suaedae sp. nov.
- The type strain is YZYP 306T (=CGMCC 1.16261T=KCTC 49101T).
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