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Pontibacter pamirensis sp. nov., isolated from saline-alkaline soil
Fang Liu1, Yang-Yang Liu1, Yu-Qian Li2
1Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi 830054, PR China.
International Journal of Systematic and Evolutionary Microbiology
|January 25, 2022
Summary
A novel red-pink bacterium, Pontibacter pamirensis TRT317^T, was discovered in saline-alkaline soil from China. Phylogenetic and genomic analyses confirm it represents a new species within the Pontibacter genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Soil ecosystems harbor diverse microbial communities.
- Saline-alkaline soils present unique environmental challenges for microbial life.
- The genus Pontibacter comprises Gram-negative bacteria with various ecological roles.
Purpose of the Study:
- To isolate and characterize a novel bacterium from saline-alkaline soil.
- To determine the taxonomic placement of the novel isolate.
- To propose a new species within the genus Pontibacter.
Main Methods:
- Isolation of bacteria from saline-alkaline soil.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- Physiological, biochemical, and chemotaxonomic characterization.
- Genomic DNA G+C content determination.
- Average Nucleotide Identity (ANI) calculation.
Main Results:
- A novel Gram-negative, aerobic, red-pink rod, strain TRT317^T, was isolated.
- Phylogenetic analysis showed highest similarity to Pontibacter species (96.3%).
- Physiological tests revealed growth at 4-40°C, pH 6.0-10.0, and up to 7% NaCl.
- Chemotaxonomic analysis identified major fatty acids, polar lipids, and whole-cell sugars.
- Genomic analysis showed a G+C content of 47.7% and an ANI value of 88.3% with P. diazotrophicus, below the species delineation threshold.
Conclusions:
- Strain TRT317^T represents a novel species within the genus Pontibacter.
- The proposed name for the novel species is Pontibacter pamirensis sp. nov.
- The type strain is TRT317^T (=CGMCC1.18690^T=KCTC 82818^T).
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