Related Experiment Video
Updated: Feb 1, 2026
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
23.2K
CRISPR/Cas-based genome engineering in natural product discovery.
Yaojun Tong1, Tilmann Weber, Sang Yup Lee
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. tiwe@biosustain.dtu.dk.
Natural Product Reports
|December 15, 2018
Summary
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system offers versatile genome editing for natural product discovery. This review explores its applications and challenges in microbes, fungi, algae, and plants.
Area of Science:
- Molecular Biology
- Biotechnology
- Genomics
Background:
- The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has revolutionized molecular biology.
- CRISPR/Cas technology is a powerful and adaptable genome editing tool.
Purpose of the Study:
- To review the applications of CRISPR/Cas genome editing in natural product discovery.
- To discuss the challenges and future prospects of CRISPR/Cas technology in this field.
Main Methods:
- Focuses on CRISPR/SpCas9 for genome editing.
- Covers applications in diverse organisms including bacteria, fungi, microalgae, and plants.
Main Results:
- CRISPR/Cas enables efficient genome editing across various microbial and plant systems.
- The technology has significant potential for discovering novel natural products.
Conclusions:
- CRISPR/Cas is a transformative tool for natural product discovery.
- Further research is needed to overcome existing challenges and fully realize its potential.
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