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Updated: Feb 1, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
Published on: September 2, 2022
Phosphorylated-CNC/modified-chitosan nanocomplexes for the stabilization of Pickering emulsions
Jiyoo Baek1, Farihah Wahid-Pedro1, Kayeon Kim1
1Department of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1, Canada.
Abstract:
Cellulose nanocrystals (CNC) are sustainable nanomaterials that possess high tensile strength, stiffness and surface functional groups suitable for various types of modifications. In this study, phosphorylated cellulose nanocrystals (P-CNC) were prepared via acid hydrolysis with phosphoric acid to decorate phosphate groups on the surface of CNC. Also, chitosan was modified with glycidyltrimethylammonium chloride (GTMAC) to improve its solubility. GTMAC-Chitosan (GCh) and phosphorylated cellulose nanocrystals (P-CNC) were complexed via ionic gelation to produce GCh-P-CNC nanoparticles under mild sonication. Although sodium tripolyphosphate (TPP) is a common cross-linking agent used with chitosan, its application is compromised by its metastable structure that resulted in the rapid release of its encapsulated compound. Therefore, phosphorylated cellulose nanocrystal was developed as a "nanoparticle" cross-linker and a Pickering emulsifier. The nanocomplexes transformed from a rod-like to hard sphere and random coil morphology with increasing ratio of GCh/P-CNC. In comparison with TPP-Chitosan emulsion, Pickering emulsion prepared using GCh-P-CNC complex was more stable over 3 months without coalescence and phase separation.
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