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Polysaccharide produced by Bacillus subtilis using burdock oligofructose as carbon source.

Lei Xu1, Yan Lu1, Yunzhe Cong1

  • 1School of Life Science, Shandong University, Qingdao 266000, PR China.

Carbohydrate Polymers
|December 17, 2018
PubMed
Summary

A novel polysaccharide, BP, derived from Bacillus subtilis fermentation of burdock, demonstrated significant anti-cancer properties. BP inhibited cancer cell proliferation and induced apoptosis, suggesting its potential as a cancer treatment.

Keywords:
ApoptosisBacillus subtilisFermented burdockLung cancerPolysaccharide structure

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Area of Science:

  • Biochemistry
  • Microbiology
  • Pharmacology

Background:

  • Burdock oligofructose is a potential substrate for microbial fermentation.
  • Bacillus subtilis is known for producing bioactive compounds.
  • Polysaccharides exhibit diverse biological activities, including anti-cancer effects.

Purpose of the Study:

  • To produce and characterize a polysaccharide (BP) from Bacillus subtilis fermented burdock oligofructose.
  • To investigate the anti-cancer activity of BP in vitro.
  • To elucidate the mechanism of action of BP against cancer cells.

Main Methods:

  • Production of polysaccharide BP by Bacillus subtilis fermentation.
  • Isolation and purification of BP using hot water extraction, ethanol precipitation, and chromatography.
  • Characterization of BP's molecular weight and monosaccharide composition.
  • In vitro assessment of BP's effects on A549 lung cancer cells, including proliferation, reactive oxygen species (ROS) production, apoptosis, and caspase-3 pathway activation.

Main Results:

  • A high molecular weight polysaccharide (1.533 × 10^7 g/mol) designated BP was successfully produced and isolated.
  • BP is primarily composed of glucose, with smaller amounts of mannose, galactose, and arabinose, featuring a specific branched structure.
  • BP demonstrated significant inhibition of A549 cancer cell proliferation and induced apoptosis by increasing ROS production.
  • BP treatment led to the upregulation of caspase-3 expression, indicating activation of the caspase-3 apoptotic pathway.

Conclusions:

  • The study successfully produced and characterized a novel polysaccharide, BP, with potent anti-cancer properties.
  • BP exhibits anti-proliferative and apoptosis-inducing effects on cancer cells through the caspase-3 pathway.
  • BP holds promise as a potential therapeutic agent or supplement for cancer treatment.