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Updated: Feb 1, 2026

Production and Detection of Reactive Oxygen Species ROS in Cancers
Published on: November 21, 2011
Modulation of reactive oxygen levels and gene expression in sensitive and resistant tumoral cells by C-phyocyanin
E Fernandes E Silva1,2, F S Figueira3,4, A P Lettnin5,6
1Laboratório de Cultura Celular, Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. star.fs@hotmail.com.
Abstract:
In this work we investigated the possible cellular changes induced by C-phycocyanin (C-PC) in erythroleukemic cells with (K562-Lucena and FEPS) and without (K562) the multidrug resistance (MDR) phenotype. The reactive oxygen levels (ROS) (evaluated by fluorimetry) were increased relative to the control in K562 and K562-Lucena cells treated with 100 µg/mL and were not increased in FEPS cells. The expression of the following genes related to resistance was evaluated by real-time PCR: COX2, ALOX5, ABCB1 and ABCC1. Treatment with 100 µg/mL of C-PC increased COX2 and ABCB1 expression in K562-Lucena cells and reduced expression of ALOX5 in K562-Lucena and FEPS cells. ROS levels appear to be involved in biological responses to C-PC in K562 and K562-Lucena cells, although expression of the genes studied here was only modified by C-PC in the K562-Lucena cell line. Thus, it is possible to suggest that C-PC modulates the expression of COX2 and ABCB1 for the K562-Lucena in a ROS-dependent manner and the expression of ALOX5 for the FEPS in a ROS-independent manner; however, more studies are needed to elucidate these mechanisms.
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