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Updated: Jan 31, 2026

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials
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Published on: July 7, 2017

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Visualising the Cardiovascular System of Embryos of Biomedical Model Organisms with High Resolution Episcopic

Wolfgang J Weninger1, Barbara Maurer-Gesek2, Lukas F Reissig3

  • 1Division of Anatomy & MIC, Medical University of Vienna, 1090 Vienna, Austria. wolfgang.weninger@meduniwien.ac.at.

Journal of Cardiovascular Development and Disease
|December 19, 2018
PubMed

The article will briefly introduce the high-resolution episcopic microscopy (HREM) technique and will focus on its potential for researching cardiovascular development and remodelling in embryos of biomedical model organisms. It will demonstrate the capacity of HREM for analysing the cardiovascular system of normally developed and genetically or experimentally malformed zebrafish, frog, chick and mouse embryos in the context of the whole specimen and will exemplarily show the possibilities HREM offers for comprehensive visualisation of the vasculature of adult human skin. Finally, it will provide examples of the successful application of HREM for identifying cardiovascular malformations in genetically altered mouse embryos produced in the deciphering the mechanisms of developmental disorders (DMDD) program.

Keywords:
3DHREMchickdevelopmental biologyembryoepiscopichigh resolution episcopic microscopyimagingmousephenotyping

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