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Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
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Characterization of a novel lytic myophage, phiA8-29, infecting Aeromonas strains

Yang He1, Zhiwei Huang1, Xixi Zhang1

  • 1State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Archives of Virology
|December 19, 2018
PubMed

Insights

A novel lytic phage, Aeromonas virus phiA8-29, was discovered in freshwater fish. This Myoviridae family phage exhibits unique genomic features, suggesting it represents a new genus of Aeromonas phages.

Area of Science:

  • Microbiology
  • Virology
  • Aquatic Pathogen Research

Background:

  • Aeromonas bacteria are significant aquatic pathogens responsible for severe fish diseases.
  • Phage therapy is a promising alternative for combating bacterial infections in aquaculture.

Purpose of the Study:

  • To isolate and characterize a novel lytic phage targeting Aeromonas bacteria.
  • To determine the genomic and morphological features of the newly discovered phage.

Main Methods:

  • Isolation of bacteriophage from freshwater fish alimentary tract.
  • Transmission electron microscopy for morphological analysis.
  • Genome sequencing and comparative genomic analysis.

Main Results:

  • Characterization of Aeromonas virus phiA8-29, a novel lytic phage.
  • Morphological classification within the Myoviridae family due to its long contractile tail.
  • Genome analysis revealed a 144,974 bp double-stranded DNA molecule with 185 open reading frames.
  • Comparative genomics showed limited similarity to existing Aeromonas phage genomes, indicating novelty.

Conclusions:

  • Aeromonas virus phiA8-29 is a newly identified lytic Myoviridae phage.
  • The phage's unique genomic characteristics suggest it represents a new genus.
  • This discovery contributes to the understanding of Aeromonas phage diversity and potential for phage therapy.

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