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Updated: Jan 31, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Published on: October 8, 2019
Evaluation of the bacterial diversity of Spanish-type chorizo during the ripening process using high-throughput
Carmen Juárez-Castelán1, Israel García-Cano1, Alejandra Escobar-Zepeda2
1Departamento de Biotecnología, Universidad Autónoma Metropolitana, Unidad Iztapalapa, San Rafael Atlixco No.186, Col. Vicentina, 09340, Iztapalapa, Ciudad de México, Mexico.
Abstract:
Spanish-type chorizo is a fermented meat product that is highly appreciated by consumers. The physicochemical properties (pH, water activity (aw), total acidity (TA), and malonaldehyde content) were evaluated in the initial meat batter and at different ripening stages. Bacterial diversity and dynamics were also examined using high-throughput sequencing. A decrease in pH and aw was observed during ripening, while the TA and malonaldehyde contents increased significantly. This data correlated with the changes in lactic acid bacteria and enterobacteria populations. Total bacterial diversity in the initial samples was represented by Proteobacteria (44%) and Firmicutes (55%) phyla, where Pseudomonas (23%), Streptococcus (21%), Acinetobacter (14%), Bacillus (13%), and Brochothrix (11%) were the most abundant genera. In contrast, Firmicutes reached the highest frequency (~89%), with Lactobacillus and Streptococcus being the most represented at the end of ripening. Lactobacillus sakei, Pediococcus acidilactici, and Weissella thailandensis were identified by 16S rRNA gene analysis and were considered potentially responsible for the stability, microbiological safety, and sensory characteristics of this product.
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