Relationships between the bioavailability of polybrominated diphenyl ethers in soils measured with female C57BL/6...

Several in vitro methods for simulating human gastrointestinal digestion have been validated for predicting the bioavailability of heavy metals, but the methods for successfully predicting the bioavailability of organic pollutants are still limited. In this study, we used an adapted fasting in vitro digestion method (Fa-VDM) from the Simulator of the Human Intestinal Microbial Ecosystem and four other in vitro methods comprising In Vitro Gastrointestinal, a physiologically-based extraction test, the unified BARGE method, and Deutsches Institut für Normung e.V. in order to measure the bioaccessibility of polybrominated diphenyl ethers (PBDEs) in soils from an e-waste dismantling town, China, with a Standard Reference Material (SRM2585) as the control. Furthermore, the bioaccessibility data were compared with the bioavailability measured using female C57BL/6 mice. The bioavailability of PBDEs in the soils and SRM2585 were 1.7% to 38.1% and 3.9% to 48.8%, respectively, and the bioaccessibility determined using Fa-VDM were 1.6-55.4% and 6.7-32.1%. There were negative and parabolic correlations between octanol/water partition coefficient for PBDEs and the bioavailability and bioaccessibility, respectively, whereas the H/C ratios and organic matter contents of the soils did not correlate with them. The bioaccessibility data determined by Fa-VDM were generally higher than those obtained using the other four methods, mainly due to the higher bile concentration and larger liquid to solid ratio in the digestion solution in Fa-VDM. There was a significant linear relationship between the results according to the in vivo and in vitro method of Fa-VDM where the slopes varied from 0.83 to 1.16 (R² > 0.73) and intercepts from 0.3%-7.7% for BDE47, 99, 100, and 153 measured using Fa-VDM, thereby indicating that the bioaccessibility assessed by this method can potentially be used to predict the bioavailability of moderately brominated congeners in soils.