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Isolation and characterization of duck adenovirus 3 circulating in China

Shaohua Shi1,2,3, Rongchang Liu1,2,3, Chunhe Wan1,2,3

  • 1Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China.

Archives of Virology
|December 20, 2018
PubMed

Insights

New duck adenovirus 3 (DAdV-3) strains caused disease in Muscovy ducklings. This study details the virus's identification, pathogenicity, and crystal-like formations in infected liver cells.

Area of Science:

  • Veterinary Virology
  • Pathogen Discovery
  • Duck Infectious Diseases

Background:

  • Infectious disease outbreaks in Muscovy ducklings have been reported.
  • Clinical signs included swelling, hemorrhagic liver, and kidneys.

Purpose of the Study:

  • To isolate and identify the causative agent of the observed disease outbreaks.
  • To investigate the pathogenicity of the identified virus in Muscovy ducklings.

Main Methods:

  • Virus isolation from diseased ducklings.
  • Identification using transmission electron microscopy (TEM) and polymerase chain reaction (PCR).
  • Sequence analysis for precise classification.
  • Pathogenicity assessment through challenge experiments.

Main Results:

  • Four adenovirus strains were isolated and identified as duck adenovirus 3 (DAdV-3).
  • DAdV-3 FJGT01 isolate caused similar gross lesions as observed clinically.
  • TEM revealed virions forming crystal lattice structures within hepatocyte nuclei.

Conclusions:

  • Duck adenovirus 3 (DAdV-3) is associated with recent outbreaks in Muscovy ducklings.
  • The study provides new insights into the epidemiology and characteristics of this duck adenovirus.
  • Findings contribute to understanding duck adenovirus B species infections.

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