Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Filtration and Urine Formation01:32

Filtration and Urine Formation

53.7K
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys.
53.7K
Glomerular Filtration: Net Filtration Pressure01:26

Glomerular Filtration: Net Filtration Pressure

8.1K
Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP).
GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal...
8.1K
Filtration00:53

Filtration

5.2K
Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...
5.2K
Plastic Deformations01:19

Plastic Deformations

465
Plastic deformation represents a fundamental concept in materials science, which explains the irreversible change in the shape of a material when it experiences stress beyond its elastic capability. This phenomenon is important in structural engineering, especially in designing and analyzing cantilever beams—structures that are securely fixed at one end and bear loads at the opposite end. When these beams are subjected to loads within their elastic range, they will return to their...
465
Plastic Deformations01:14

Plastic Deformations

443
It is essential to understand how structural members behave under plastic deformation when the bending stress exceeds the material's yield strength. This state of deformation permanently alters the shape of the member, in contrast to the linear elastic behavior observed before yielding. The strain at any point in the member is expressed in terms of maximum strain. Notably, the neutral axis, which coincides with the centroid during elastic bending, shifts away from the centroid under plastic...
443
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

1.1K
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical outcomes following spinal fusion for cervical versus noncervical fractures in ankylosing spondylitis and DISH.

North American Spine Society journal·2026
Same author

Is Parkinson Disease a Risk Factor After Total Elbow Arthroplasty? Midterm Survivorship in a Matched Cohort.

Journal of hand surgery global online·2026
Same author

Paget's Disease of the Spine as a Catalyst for Osteosarcoma: A Narrative Review.

Cureus·2026
Same author

Hypoplasia of the Atlas Does Not Correlate With Subaxial Canal Stenosis: A Retrospective Cohort Study.

Cureus·2026
Same author

Redefining lightweight vision models for healthcare AI.

Frontiers in artificial intelligence·2026
Same author

Disparities in Depression Among Older Asian, Native Hawaiian, and Pacific Islander Patients With Lung Cancer.

Cancer medicine·2026
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.0K

A scalable filtration method for high throughput screening based on cell deformability.

Navjot Kaur Gill1, Chau Ly, Kendra D Nyberg

  • 1Department of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, California, USA. rowat@ucla.edu.

Lab on a Chip
|December 20, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a scalable cell filtration device for high-throughput screening of cell deformability, a key biomarker for cell state and disease progression. The method effectively differentiates cancer cells based on their mechanical properties.

More Related Videos

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
08:49

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Published on: March 20, 2018

9.4K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.7K

Related Experiment Videos

Last Updated: Jan 31, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

10.0K
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
08:49

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Published on: March 20, 2018

9.4K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.7K

Area of Science:

  • Biophysics
  • Cell Biology
  • Cancer Research

Background:

  • Cell deformability is a crucial, label-free biomarker for cell state, relevant in conditions from stem cell differentiation to cancer.
  • Existing methods for measuring cell deformability lack the scalability required for high-throughput screening applications.

Purpose of the Study:

  • To develop and validate a scalable cell filtration device for high-throughput measurement of cell deformability.
  • To demonstrate the utility of this device in distinguishing between different cancer cell states and responses to treatment.

Main Methods:

  • A novel cell filtration device utilizing pressure-driven cell deformation through pillar arrays in a 96-well format was designed using soft lithography.
  • Filtrate volume, correlating with cell deformability, was measured using a standard plate reader.
  • The device was validated using human ovarian cancer cells with acquired cisplatin-resistance and various other cancer cell lines (ovarian, breast, prostate).

Main Results:

  • The filtration device successfully differentiated cisplatin-resistant ovarian cancer cells from sensitive ones, consistent with quantitative deformability cytometry.
  • Differential filtration was observed across various cancer cell lines, correlating with metastatic potential and epithelial-to-mesenchymal transition markers (Snail, Slug).
  • Drug treatments known to affect the cytoskeleton and nucleus altered ovarian cancer cell filtration, demonstrating sensitivity to cellular perturbations.

Conclusions:

  • The developed scalable filtration device offers a high-throughput, label-free method for assessing cell deformability.
  • This technology has significant potential for screening cell states, drug responses, and disease progression in various cancer types.