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Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish
Jolly M Saju1, Mohammad Sorowar Hossain2, Woei Chang Liew1
1Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore.
Cell Reports
|December 20, 2018
Summary
Heat shock factor 5 (Hsf5) is crucial for male fertility in zebrafish. Loss of Hsf5 causes infertility by disrupting meiotic progression and sperm development during spermatogenesis.
Area of Science:
- Molecular Biology
- Genetics
- Reproductive Biology
Background:
- Heat shock factors (Hsfs) are transcription factors regulating cellular responses to stress.
- Known Hsfs (Hsf1-4) are involved in stress, development, and gametogenesis.
- Hsf5, a less-studied Hsf member, is investigated here.
Purpose of the Study:
- To investigate the function of Hsf5 in zebrafish.
- To determine Hsf5's role in spermatogenesis and male fertility.
Main Methods:
- CRISPR/Cas9 gene editing to create hsf5 knockout zebrafish.
- Phenotypic analysis of mutant zebrafish reproductive systems.
- Gene expression analysis in hsf5 mutants.
Main Results:
- hsf5 knockout males were infertile with reduced sperm count and abnormal sperm morphology.
- Hsf5 deficiency caused cell cycle arrest in meiotic prophase I and increased apoptosis.
- Significant misregulation of genes involved in cell cycle, apoptosis, and signal transduction was observed.
Conclusions:
- Hsf5 is essential for male fertility and proper meiotic progression in zebrafish spermatogenesis.
- Hsf5 regulates key genes involved in early spermatogenesis.
- Hsf5 plays a critical role in maintaining male reproductive health.
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