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Oligomer based real-time detection of microorganisms producing nuclease enzymes
Erkan Mozioğlu1, Sema Akyürek2, Simay Gündüz3
1Bioanalysis Laboratory, Chemistry Group, National Metrology Institute, The Scientific and Technological Research Council of Turkey (TÜBİTAK-UME), Gebze, Turkey. erkanmozioglu@yahoo.com erkan.mozioglu@acibadem.edu.tr and Medical Biotechnology Program, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
Abstract:
In this study, we provide a method using fluorescently labeled oligonucleotides for the diagnosis of microorganisms producing nucleases in real time, while growing them in culture media. The detection of such microorganisms was possible in a short period of time, as short as 10 minutes up to a maximum of 8 hours, depending on the bacterial density. We also showed the suitability of this new method for determination of minimum inhibitory concentration (MIC) in culture media in a very short period of time, compared to conventional methods. We believe that it can make a significant contribution to gain new insights for analysis of complex materials such as clinical samples, food samples and environmental samples.
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