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Updated: Jan 31, 2026
08:05
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
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Elevated S100A4 in asthmatics and an allergen-induced mouse asthma model
Xiaolin Huang1, Dongming Qu2, Yue Liang3
1The Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, China.
Journal of Cellular Biochemistry
|December 21, 2018
Summary
Elevated S100A4 protein levels in sputum are linked to asthma severity. Targeting S100A4 with antibodies reduced airway inflammation and hyper-responsiveness in an asthma mouse model.
Area of Science:
- Pulmonary Medicine
- Immunology
- Molecular Biology
Background:
- S100A4 protein levels are elevated in certain inflammatory conditions.
- The role and expression of S100A4 in asthma pathogenesis remain largely uncharacterized.
Purpose of the Study:
- To investigate the expression and function of S100A4 in asthma.
- To determine if S100A4 contributes to airway inflammation and hyper-responsiveness.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure S100A4 levels in sputum and plasma of healthy controls and asthmatic patients.
- An allergen-induced asthma mouse model was utilized, with some mice treated with an anti-S100A4 antibody.
- Lung tissue and bronchoalveolar lavage fluid (BALF) were analyzed for S100A4 expression and inflammatory markers.
Main Results:
- Asthmatic patients showed significantly higher S100A4 levels in sputum, correlating negatively with lung function and positively with sputum eosinophils and lymphocytes.
- The asthma mouse model exhibited increased S100A4 expression in the lungs and BALF.
- Treatment with anti-S100A4 antibody reduced inflammatory cell infiltration, mediators, and airway hyper-responsiveness in the mouse model.
- PI3K inhibition with LY294002 decreased S100A4 expression and secretion in asthmatic mice.
Conclusions:
- S100A4 is upregulated in asthma and may play a significant role in airway inflammation.
- Targeting S100A4 presents a potential therapeutic strategy for managing asthma.
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