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Updated: Jan 31, 2026

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
Published on: April 30, 2012
Regulation of the Distribution of Cells in Mixed Spheroids by Altering Migration Direction
Yuya Mizukami1, Yuki Takahashi1, Kazunori Shimizu2
11 Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
Impact Statement:
Complex and functional artificial tissues consisting of multiple types of cells are generally required. However, few reliable methods to control the cellular distribution have been developed. Our present study has revealed how the core-shell type distribution is formed in NIH3T3/MIN6 spheroids. We demonstrated that focal adhesion kinase signal was the key for the cell localization in the spheroids. Moreover, we succeeded in regulating their distribution based on the mechanism revealed in the present study. These novel findings will provide a new approach for constructing artificial tissues with proper cell arrangement, which would be suitable for tissue engineering.
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