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Tumorigenic transformation of mouse NIH 3T3 cells by transfection with plasmid pSV2neo
J S Wallace1, K A Fleming, D Tarin
1Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, U.K.
The Journal of Pathology
|September 1, 1988
Abstract:
This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co-transfection and subsequent selection with neomycin.