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MicroRNA Dysregulation Associated with Red Blood Cell Storage

Xiaojie Chen1, Xuhong Xie1, Yanfen Xing1

  • 1Department of Blood Transfusion, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur Transfusionsmedizin Und Immunhamatologie
|December 22, 2018
PubMed
Abstract

Insights

Dysregulated microRNAs (miRNAs) were identified in stored red blood cells (RBCs), suggesting they could serve as biomarkers for assessing RBC storage quality and damage.

Area of Science:

  • Hematology
  • Molecular Biology
  • Biochemistry

Background:

  • Stored red blood cells (RBCs) experience storage lesions, leading to detrimental changes.
  • MicroRNAs (miRNAs) play crucial roles in cellular processes like apoptosis.

Purpose of the Study:

  • To investigate the potential involvement of miRNAs in the damage mechanisms of stored RBCs.
  • To identify specific miRNAs that are altered during RBC storage.

Main Methods:

  • Collected blood from healthy donors.
  • Separated leuko-reduced RBCs into fresh and 20-day stored groups.
  • Analyzed miRNA expression profiles.

Main Results:

  • Eight miRNAs showed dysregulated expression in 20-day stored RBCs compared to fresh RBCs.
  • These dysregulated miRNAs are linked to apoptosis and senescence pathways.
  • Specific miRNAs (e.g., miR-31-5p, miR-196a-5p) were found to be increased, while others (e.g., miR-96-5P, miR-150-5P) were decreased.

Conclusions:

  • Altered miRNA expression in stored RBCs may indicate storage lesions.
  • Detection of these dysregulated miRNAs could serve as potential biomarkers for evaluating RBC quality.
Keywords:
HemorheologyStorage lesionStored RBCsmiRNA dysregulation

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