Related Experiment Video

Updated: Jan 31, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.9K

MicroRNA-1294 targets HOXA9 and has a tumor suppressive role in osteosarcoma

Z-F Zhang1, G-R Li, C-N Cao

  • 1Department of Joint Surgery, Yantai Yuhuangding Hospital, Yantai, Zhifu District, Yantai, Shandong, P. R. China. jiangxf_doctor@126.com.

European Review for Medical and Pharmacological Sciences
|December 22, 2018
PubMed
Abstract

Insights

MicroRNA-1294 (miR-1294) is downregulated in osteosarcoma (OS), suppressing tumor growth and invasion by targeting HOXA9. This finding highlights miR-1294 as a potential therapeutic target for OS patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-1294 (miR-1294) exhibits tumor-suppressive roles in various cancers.
  • The specific function of miR-1294 in osteosarcoma (OS) remains unexplored.

Purpose of the Study:

  • To investigate the clinical significance of miR-1294 in osteosarcoma.
  • To elucidate the underlying molecular mechanisms of miR-1294 action in OS progression.

Main Methods:

  • Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) for miR-1294 expression analysis.
  • Luciferase reporter assays and Western blot to identify and validate miR-1294 targets.
  • In vitro functional assays (proliferation, invasion) to assess miR-1294 and HOXA9 effects.

Main Results:

  • miR-1294 was significantly downregulated in OS tissues and cell lines.
  • Lower miR-1294 levels correlated with reduced overall survival in OS patients.
  • miR-1294 overexpression inhibited OS cell proliferation and invasion; HOXA9 was identified as a direct target, and its overexpression reversed these inhibitory effects.

Conclusions:

  • miR-1294 acts as a tumor suppressor in osteosarcoma.
  • The miR-1294/HOXA9 axis plays a crucial role in OS progression.
  • miR-1294 represents a potential therapeutic biomarker for osteosarcoma.

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Role of Septins01:02

Role of Septins

Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
2.2K
The Role of Culture01:23

The Role of Culture

Culture plays a crucial role in shaping self-identity and influencing thought and behavior, a foundational interest within social psychology. The multicultural perspective recognizes that individuals do not exist in a vacuum; instead, their experiences, perceptions, and actions are deeply influenced by the intersecting dimensions of their cultural, ethnic, and social group affiliations.Cultural Influence on Self-Identity and Social PerceptionCultural frameworks inform how individuals define...
417
Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids01:31

Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids

In the complex environment of the gastric lumen, excessive acid secretion can lead to the formation or worsening of ulcers within the delicate mucosal layer. Antacids, such as sodium bicarbonate and calcium carbonate, provide relief by neutralizing this acid, transforming it into harmless salt and water. This neutralization process raises the gastric pH from a highly acidic level of 1 to a more basic 3-4, reducing the acidity within the stomach.
However, this neutralization reaction between...
700
Role-Based Identity01:21

Role-Based Identity

Role-based identities are central to understanding how individuals navigate social environments by adopting distinct self-conceptions aligned with various societal roles. These identities are not fixed traits but are constructed through personal actions and the social feedback individuals receive in context-specific interactions. Each social role, such as student, teacher, or friend, carries a set of expectations and norms that influence how people think, feel, and behave within that...
204