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Updated: Jan 31, 2026

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
Published on: September 6, 2012
Combinatorial Synthesis of Peptoid Arrays via Laser-Based Stacking of Multiple Polymer Nanolayers
Daniela S Mattes1,2, Bettina Streit1,3, Dhaka R Bhandari4
1Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Abstract:
Here, the combinatorial synthesis of molecule arrays via a laser-assisted process is reported. Laser-transferred polymer nanolayers with embedded monomers, activators, or bases can be reliably stacked on top of each other, spot-by-spot, to synthesize molecule arrays. These various chemicals in the nanometer-thin layers are mixed by heat or solvent vapor, inducing coupling reactions. As an example, peptoid arrays with a density of 10 000 spots per cm2 with the sub-monomer or monomer method are generated. Moreover, successful reactions spot-by-spot are verified by laser-transferring MALDI-matrix (Matrix-assisted laser desorption/ionization) followed by MALDI mass spectrometry imaging.
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