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Updated: Jan 31, 2026
13:18
Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status
Published on: May 18, 2019
12.6K
A versatile Rapture (RAD-Capture) platform for genotyping marine turtles.
Lisa M Komoroske1,2, Michael R Miller3, Sean M O'Rourke3
1Department of Environmental Conservation, University of Massachusetts Amherst, Amherst, Massachusetts.
Molecular Ecology Resources
|December 22, 2018
Summary
A new high-throughput sequencing (HTS) genotyping platform for marine turtles offers adaptable and affordable methods for conservation genetics. This tool enables large-scale genetic studies, aiding in population structure and management for these vulnerable species.
Area of Science:
- Conservation Genetics
- Genomics
- Marine Biology
Background:
- High-throughput sequencing (HTS) advances wildlife genetic studies.
- Tailored HTS methods are needed for specific organisms like marine turtles.
- Mobile species conservation requires affordable, adaptable HTS genotyping.
Purpose of the Study:
- To develop a versatile HTS targeted enrichment platform for marine turtles.
- To adapt the Rapture (RAD-Capture) method for marine turtle conservation genetics.
- To enable large-scale, comparable genetic analyses across spatial and temporal scales.
Main Methods:
- Adaptation of the Rapture (RAD-Capture) method for marine turtle HTS genotyping.
- Targeted enrichment of specific genomic regions.
- Multiplexing and genotyping of over 1,000 samples.
Main Results:
- Consistent enrichment of targeted genomic regions across species.
- Discovery of candidate variants for conservation genetics applications.
- Successful species identification and genotyping of numerous samples.
- Identification of informative SNPs for stock structure and population assignment in green turtles.
Conclusions:
- The developed HTS platform is a versatile tool for marine turtle genetic studies.
- The platform supports large-scale initiatives like global genetic databases.
- Methodological improvements are identified for low-concentration samples.
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