Related Experiment Video
Updated: Jan 31, 2026
05:53
An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury
Published on: July 15, 2021
8.3K
Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats
T Ćelić1, H Omrčen1, J Španjol2
1Department of Anatomy, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
Transplantation Proceedings
|December 23, 2018
Summary
Recombinant human bone morphogenetic protein-7 (rhBMP-7) protects kidneys from cold ischemic injury by preserving tissue morphology and preventing cell transformation. This offers a promising strategy to improve kidney graft preservation and reduce delayed graft function.
Area of Science:
- Nephrology
- Regenerative Medicine
- Transplantation Immunology
Background:
- Deceased donor kidneys face cold ischemic injury during hypothermic preservation, leading to delayed graft function.
- Bone morphogenetic protein-7 (BMP-7) shows potential in tissue regeneration and preservation under ischemic conditions.
Purpose of the Study:
- To investigate the protective effects of recombinant human BMP-7 (rhBMP-7) on kidney grafts during cold ischemia.
Main Methods:
- An experimental rat model of kidney cold ischemia was used.
- Kidneys were perfused with University of Wisconsin (UW) solution, rhBMP-7, or rhBMP-7 + UW solution.
- Kidneys were subjected to cold ischemia for 6, 12, and 24 hours.
Main Results:
- rhBMP-7 perfusion preserved kidney tissue morphology better than UW solution after 24 hours of cold ischemia.
- BMP-7 and E-cadherin expression increased in tubular epithelial cells with rhBMP-7 treatment.
- Transforming growth factor-β and α-smooth muscle actin expression decreased with rhBMP-7 treatment.
- Statistically higher Smad1, Smad5, and Smad8 mRNA levels were observed in kidneys perfused with rhBMP-7.
Conclusions:
- rhBMP-7 effectively preserves kidney graft morphology during cold ischemia.
- BMP-7 prevents epithelial-to-mesenchymal transition, maintaining the epithelial phenotype of tubular cells.
- rhBMP-7 demonstrates potential as a therapeutic agent to improve kidney graft preservation.
Related Concept Videos
01:58Mechanical Protein Functions
5.6K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.6K
02:45Responses to Heat and Cold Stress
14.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
01:58Mechanical Protein Function
2.5K
2.5K
01:51Mechanisms of Retrovirus-induced Cancers
7.0K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
7.0K
01:27Cold Weather Concreting
375
When freshly poured concrete is exposed to freezing temperatures before it has set, the water within the concrete can freeze. This expansion disrupts the setting process, delays chemical reactions necessary for hardening, and increases the volume of pores within the hardened concrete, which weakens its overall structure. If the concrete manages to reach an appreciable strength before it freezes, the damage can be somewhat mitigated.
To counteract the negative impacts of cold weather, ensuring...
To counteract the negative impacts of cold weather, ensuring...
375
01:30Transducer Mechanism: G Protein–Coupled Receptors
4.1K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
GPCRs are also called heptahelical,...
4.1K