Related Experiment Video
Updated: Jan 31, 2026
13:00
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Published on: April 4, 2014
21.4K
Characterizing Drug-Polymer Bead Interactions Using Isothermal Titration Calorimetry
Tanya S Swaine1, Pedro Garcia2, Yiqing Tang2
1School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK.
Journal of Pharmaceutical Sciences
|December 23, 2018
Summary
Drug-eluting bead properties significantly impact chemotherapy delivery. Microsphere size affects binding rate, while drug characteristics and bead type influence binding stoichiometry and kinetics for cancer therapy.
Area of Science:
- Oncology
- Materials Science
- Pharmacology
Background:
- Drug-eluting beads are crucial for targeted cancer therapy.
- Understanding drug-microsphere interactions is key to optimizing treatment efficacy.
- Commercially available embolization microspheres vary in size and material composition.
Purpose of the Study:
- To investigate the thermodynamic and kinetic binding of four chemotherapy drugs with various embolization microspheres.
- To determine the impact of microsphere size and type on drug binding characteristics.
- To elucidate the influence of drug properties on binding interactions.
Main Methods:
- Isothermal titration calorimetry (ITC) was employed to study drug-microsphere binding.
- Four clinically relevant drugs (doxorubicin, irinotecan, mitoxantrone, topotecan) were tested.
- Two types of microspheres (DC BeadM1™ and LifePearl™) in various sizes were utilized.
Main Results:
- Drug-to-sulfonate group molar ratios varied, indicating drug shape, charge, and size influence binding.
- Microsphere size did not affect binding stoichiometry but was inversely proportional to binding rate.
- Doxorubicin binding was faster with polyethylene glycol hydrogel beads compared to polyvinylalcohol hydrogel beads at specific ratios.
Conclusions:
- Drug-eluting bead characteristics significantly modulate chemotherapy binding kinetics and thermodynamics.
- Optimizing microsphere properties can enhance drug delivery efficiency in interventional oncology.
- Further research into drug-material interactions is essential for advancing targeted cancer therapies.
Related Concept Videos
03:02Constant Pressure Calorimetry
97.7K
Calorimetry is a technique used to measure the amount of heat involved in a chemical or physical process or to measure the heat transferred to or from a substance. The heat is exchanged with a calibrated and insulated device called the calorimeter. Calorimetry experiments are based on the assumption that there is no heat exchange between the insulated calorimeter and the external environment. The well-insulated calorimeters prevent the transfer of heat between the calorimeter and its external...
97.7K
01:19Calorimetry
4.5K
When objects at different temperatures are placed in contact with each other but isolated from everything else, they attain thermal equilibrium. A container that prevents heat transfer in or out is called a calorimeter, and the use of a calorimeter to make measurements is called calorimetry. Generally, these measurements involve heat or specific heat capacity. The term "calorimetry problem" is used for any problem where the specified objects are thermally isolated from their...
4.5K
02:34Polymers
40.8K
The word polymer is derived from the Greek words “poly” which means “many” and “mer” which means “parts”. Polymers are long chains of molecules composed of repeating units of smaller molecules, known as monomers. They either occur naturally, such as DNA and proteins, or can be constructed synthetically, like plastics. They have varied structural characteristics, such as linear chains, branched chains, or complex networks, that contribute to the...
40.8K
01:25Pharmacokinetics: Drug–Drug Interactions
440
Drug interactions occur when the pharmacological effect of one drug is altered by another substance, either enhancing or diminishing its activity. The drug whose activity is altered is known as the object drug, and the substance causing the alteration is called the agent drug or the precipitant. The net effects of these interactions are mostly undesirable, leading to decreased effectiveness or increased adverse effects. In rare cases, interactions can be beneficial, such as the enhanced...
440
02:08Titration of a Polyprotic Acid
105.2K
A polyprotic acid contains more than one ionizable hydrogen and undergoes a stepwise ionization process. If the acid dissociation constants of the ionizable protons differ sufficiently from each other, then the titration curve for such polyprotic acid generates a distinct equivalence point for each of its ionizable hydrogens. Therefore, titration of a diprotic acid results in the formation of two equivalence points, whereas the titration of a triprotic acid results in the formation of three...
105.2K
01:29Drug-Receptor Interactions
7.5K
Drug-receptor interaction describes the binding of receptors by drugs, but not all drug-receptor interactions result in activation and tissue response. For instance, the binding of agonists activates the receptor to generate a cellular reaction, while antagonists bind to receptors without causing their activation.
Several parameters, such as the drug's affinity for its receptor and its efficacy, which is its ability to activate the receptor, determine the drug's effect on the tissue....
Several parameters, such as the drug's affinity for its receptor and its efficacy, which is its ability to activate the receptor, determine the drug's effect on the tissue....
7.5K