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Cell-Free DNA: An Overview of Sample Types and Isolation Procedures
1Department of Medical Biotechnologies, University of Siena, Siena, Italy. filippo.martignano@gmail.com.
Abstract:
The study of cell-free DNA (cfDNA) is often challenging due to genomic DNA contamination, low concentration, and high fragmentation. Therefore, it is important to optimize pre-analytical and analytical procedures in order to maximize the performance of cfDNA-based analyses.In this chapter, we report the most common methods for the correct collection, centrifugation, storage, and DNA isolation from cell-free biological sources such as plasma, urines, cerebrospinal fluid, and pleural effusion fluid.
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