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Related Experiment Video

Updated: Jan 31, 2026

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography

Published on: September 5, 2012

An Ethanol-Free Autologous Thrombin System.

Andrea M Matuska¹, Marina K Klimovich¹, John R Chapman¹

¹Arthrex, Inc., Naples, Florida; and.

The Journal of Extra-Corporeal Technology

|December 25, 2018

Summary

A novel point-of-care device prepares ethanol-free autologous serum (AS) with significant thrombin activity. This system offers a safer, reliable alternative for hemostasis and wound healing applications.

Keywords:

autologous blood-coagulation device fibrin platelet rich plasma thrombin

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Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

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Published on: September 22, 2012

Area of Science:

Biochemistry
Biomedical Engineering
Regenerative Medicine

Background:

Thrombin is crucial for hemostasis and wound healing.
Autologous thrombin avoids pathogen transmission and immune responses.
Existing methods use ethanol, posing cytotoxicity risks.

Purpose of the Study:

To evaluate a new point-of-care device for preparing ethanol-free autologous serum (AS).
To assess the reliability and flexibility of the Thrombinator™ System.
To determine the thrombin activity and bone graft scaffold formation capabilities.

Main Methods:

The Thrombinator™ System was used to prepare AS from whole blood (WB) and platelet-poor plasma (PPP).
120 devices were tested across 30 healthy donors.
Thrombin activity was measured using a coagulation analyzer; scaffold cohesion was assessed with a durometer.

Main Results:

Average thrombin activity was 20.6 ± 2.7 IU/mL from WB and 13.4 ± 3.8 IU/mL from PPP.
Clot times were 3.9 seconds (WB) and 5.9 seconds (PPP).
The system demonstrated reliability, flexibility with varying blood volumes, and consistent production over four cycles.

Conclusions:

The Thrombinator™ System reliably produces ethanol-free autologous serum with significant thrombin activity.
This method offers a safer alternative to ethanol-based preparations for hemostasis and regenerative medicine.
Cohesive bone graft scaffolds were consistently formed when AS was combined with other materials.