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Related Experiment Video

Updated: Aug 27, 2025

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
09:14

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Published on: October 20, 2019

9.9K

Micro-fragmented adipose tissue cellular composition varies by processing device and analytical method.

Valerie Greenwood1, Peter Clausen2, Andrea M Matuska2

  • 1Arthrex Inc, One Arthrex Way, Naples, FL, 34108, USA. Valerie.Greenwood@Arthrex.com.

Scientific Reports
|September 27, 2022
PubMed
Summary

Micro-fragmented adipose tissue (MFAT) processing affects cell content and function. The AutoPose system yielded higher mesenchymal stromal cell (MSC) concentrations with beneficial anti-inflammatory properties compared to other methods.

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Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Tissue Engineering

Background:

  • Autologous adipose-derived biologics are clinically relevant due to accessible progenitor and immunomodulatory cells.
  • Micro-fragmented adipose tissue (MFAT) preserves native cellular niches, potentially outperforming enzymatically processed stromal vascular fraction (SVF).
  • Standardization issues hinder direct comparison of MFAT products from different processing systems.

Purpose of the Study:

  • To analyze and compare the cellular content and cytokine release of MFAT processed by LipoGems® and AutoPose™ Restore systems.
  • To evaluate different techniques for assessing MFAT graft cellularity.
  • To understand how processing methods influence the composition and potential therapeutic properties of adipose-derived biologics.

Main Methods:

  • Flow cytometry analysis of SVF and MFAT outgrowth populations.
  • Culture of MFAT to assess adherent cell migration.
  • Assessment of cytokine release profiles during cell culture.
  • Comparison of MFAT from LipoGems® and AutoPose™ Restore systems.

Main Results:

  • SVF exhibited more diverse progenitor populations than MFAT outgrowth.
  • MFAT outgrowth showed lower cell concentrations, primarily mesenchymal stromal cells (MSCs).
  • The AutoPose system yielded significantly higher MSC concentrations in MFAT, with enhanced anti-inflammatory and reduced inflammatory cytokine secretion.

Conclusions:

  • MFAT cellularity and characteristics are significantly influenced by processing methods.
  • Different analytical techniques can effectively evaluate MFAT graft cellularity.
  • The AutoPose system demonstrates potential for producing MFAT with favorable cellular and immunomodulatory profiles for therapeutic applications.