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MicroRNA miR-252 targets mbt to control the developmental growth of Drosophila

D-H Lim1,2, S Lee1,2, J Y Han1

  • 1College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, South Korea.

Insect Molecular Biology
|December 25, 2018
PubMed

Insights

MicroRNAs (miRNAs) regulate development. Overexpressing miR-252 in Drosophila larvae reduced growth and caused developmental delays. This highlights the ecdysone/miR-252/mbt pathway

Area of Science:

  • Developmental biology
  • Genetics
  • Molecular biology

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression in animal development.
  • The ecdysone-responsive miRNA, miR-252, is typically upregulated during later Drosophila development stages.
  • Understanding miRNA roles is crucial for deciphering developmental processes.

Purpose of the Study:

  • To investigate the function of miR-252 in Drosophila larval and adult development.
  • To identify the molecular targets and regulatory pathways involving miR-252.
  • To elucidate the role of the ecdysone/miR-252/mbt axis in growth control.

Main Methods:

  • Overexpression of miR-252 in larval fat body.
  • Analysis of larval and adult size, cell size, and cell number.
  • Assessment of developmental transitions (larval-to-pupal).
  • Gene target identification and validation (mbt).
  • Rescue experiments with mbt co-overexpression.

Main Results:

  • miR-252 overexpression decreased larval tissue mass by reducing cell size and number, leading to smaller larvae.
  • Overexpression caused delayed pupal transition and defective spiracle eversion, with reduced adult size and mass.
  • Loss of miR-252 in adults resulted in increased mass.
  • miR-252 directly targets and represses mbt, a p21-activated kinase.
  • Co-overexpression of mbt rescued miR-252-induced developmental and growth defects.

Conclusions:

  • The ecdysone/miR-252/mbt regulatory axis is critical for controlling growth during Drosophila development.
  • miR-252 acts as a negative regulator of growth by targeting mbt.
  • Dysregulation of miR-252 impacts multiple developmental stages and overall organismal size.
Keywords:
DrosophilambtgrowthmiR-252

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