Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K
Epigenetic Regulation01:46

Epigenetic Regulation

33.7K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.7K
Turnover Number and Catalytic Efficiency01:19

Turnover Number and Catalytic Efficiency

21.5K
The turnover number of an enzyme is the maximum number of substrate molecules it can transform per unit time. Turnover numbers for most enzymes range from 1 to 1000 molecules per second. Catalase has the known highest turnover number, capable of converting up to 2.8×106 molecules of hydrogen peroxide into water and oxygen per second. Lysozyme has the lowest known turnover number of half a molecule per second.
Chymotrypsin is a pancreatic enzyme that breaks down proteins during digestion....
21.5K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

5.1K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
5.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026
Same author

DNA Framework Nucleator-Enabled Intelligent Hydrogel Interfaces on Living Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Zeta potential transition correlates with optimal DNA origami silicification temperature.

Chemical communications (Cambridge, England)·2026
Same author

An Integrated Peroxidase-like Nanozyme for Photothermally Enhanced Catalytic Antibacterial Treatment in Multiple Oral Diseases.

ACS applied materials & interfaces·2026
Same author

Bifunctional Fe-Doped MoS<sub>2</sub> Nanozyme for DNA-Engineered Protein Discrimination and Photothermally Enhanced Bacterial Eradication.

ACS applied materials & interfaces·2026
Same author

Molecular tuning of DNA framework-programmed silicification by cationic silica cluster attachment.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jan 31, 2026

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction
09:28

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction

Published on: August 31, 2018

7.7K

Engineering Nanozymes Using DNA for Catalytic Regulation.

Caixia Zeng1, Na Lu1, Yanli Wen2

  • 1School of Materials Engineering , Shanghai University of Engineering Science , Shanghai 201620 , China.

ACS Applied Materials & Interfaces
|December 25, 2018
PubMed
Summary

DNA engineering regulates nanozyme catalysis. Hybridization chain reaction (HCR) products significantly enhance nanozyme activity, enabling sensitive detection of DNA sequences and single nucleotide polymorphism discrimination.

Keywords:
DNAHCR amplificationcatalytic regulationcolorimetric biosensornanozymes

More Related Videos

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

7.0K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

946

Related Experiment Videos

Last Updated: Jan 31, 2026

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction
09:28

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction

Published on: August 31, 2018

7.7K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

7.0K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

946

Area of Science:

  • Nanomaterials Science
  • Biotechnology
  • Catalysis

Background:

  • DNA interactions with nanomaterials are crucial for advanced material design.
  • Limited research exists on DNA's impact on nanozyme peroxidase-like activity.
  • Nanozymes offer tunable catalytic properties for various applications.

Purpose of the Study:

  • To investigate DNA structures as capping ligands for nanozyme catalytic activity regulation.
  • To explore the influence of different DNA structures on nanozyme peroxidase-like properties.
  • To develop a DNA-based sensing strategy utilizing enhanced nanozyme activity.

Main Methods:

  • Engineering bio-nanointerfaces using various DNA structures (dsDNA, ssDNA, hairpin DNA, HCR products) on nanozymes.
  • Systematic investigation of parameters affecting HCR amplification and nanozyme catalysis (DNA concentration, ionic strength, nanozyme amount).
  • Development of a label-free colorimetric sensing strategy based on HCR-amplified nanozyme activity.

Main Results:

  • HCR product-treated nanozymes exhibited the highest peroxidase-like activity compared to other DNA structures.
  • Catalytic activity was modulated by DNA structure, concentration, ionic strength, and nanozyme amount.
  • A sensitive colorimetric assay for Yersinia pestis DNA detection (100 pM limit) and SNP discrimination was established.

Conclusions:

  • DNA engineering provides a facile strategy to precisely regulate nanozyme catalytic activity.
  • Understanding DNA-nano metal nanoparticle interactions is key for biosensing development.
  • HCR amplification offers a powerful tool for enhancing nanozyme-based biosensing capabilities.