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Updated: Jan 31, 2026
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Gold Nanoparticle Synthesis
Published on: July 10, 2021
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Gold nanoparticles of different shape for bicolor lateral flow test
Alina V Petrakova1, Alexandr E Urusov1, Anatoly V Zherdev1
1A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Analytical Biochemistry
|December 25, 2018
Summary
This study introduces a novel lateral flow assay using differently colored gold nanoparticles to prevent misinterpretation of results. Blue nanoflowers and red nanospheres improve test strip clarity and accuracy in toxin detection.
Area of Science:
- Nanotechnology
- Biotechnology
- Analytical Chemistry
Background:
- Spherical gold nanoparticles are standard markers in lateral flow assays.
- Identical coloration in test and control zones can cause interpretation errors.
Purpose of the Study:
- To develop an immunochromatographic test strip with distinct color zones for improved accuracy.
- To utilize gold nanoparticles of different shapes and colors for enhanced visual differentiation.
Main Methods:
- Synthesized blue nanoflower and red nanosphere gold nanoparticles.
- Conjugated nanoparticles with antibodies for indirect labeling.
- Designed a test strip with separate colored zones for test and control lines.
Main Results:
- Achieved an instrumental detection limit of 30 pg/mL for T-2 toxin.
- Established a working range of 0.06–0.9 ng/mL for T-2 toxin detection.
- Successfully combined differently shaped nanoparticles for improved assay interpretation.
Conclusions:
- The novel test strip design with distinct nanoparticle colors enhances assay reliability.
- This approach offers a clearer visual interpretation, reducing diagnostic errors.
- Demonstrated the efficacy of using shape-differentiated nanoparticles in lateral flow assays.
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