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Updated: Jan 31, 2026

In vivo Measurement of Knee Extensor Muscle Function in Mice
Published on: March 4, 2021
Agreement of measured and calculated muscle activity during highly dynamic movements modelled with a spherical knee
Thomas Dupré1, Mareike Dietzsch1, Igor Komnik1
1Institute of Biomechanics and Orthopaedics, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.
Abstract:
The inclusion of muscle forces into the analysis of joint contact forces has improved their accuracy. But it has not been validated if such force and activity calculations are valid during highly dynamic multidirectional movements. The purpose of this study was to validate calculated muscle activation of a lower extremity model with a spherical knee joint for running, sprinting and 90°-cutting. Kinematics, kinetics and lower limb muscle activation of ten participants were investigated in a 3D motion capture setup including EMG. A lower extremity rigid body model was used to calculate the activation of these muscles with an inverse dynamics approach and a cubic cost function. Correlation coefficients were calculated to compare measured and calculated activation. The results showed good correlation of the modelled and calculated data with a few exceptions. The highest average correlations were found during walking (r = 0.81) and the lowest during cutting (r = 0.57). Tibialis anterior had the lowest average correlation (r = 0.33) over all movements while gastrocnemius medius had the highest correlation (r = 0.9). The implementation of a spherical knee joint increased the agreement between measured and modelled activation compared to studies using a hinge joint knee. Although some stabilizing muscles showed low correlations during dynamic movements, the investigated model calculates muscle activity sufficiently.
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