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New Insights on the Mechanisms Affecting Fertility in Men with Non-Seminoma Testicular Cancer before Cancer Therapy

Tania R Dias1,2,3, Ashok Agarwal4, Peter N Pushparaj5

  • 1American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.

The World Journal of Men'S Health
|December 28, 2018
PubMed
Abstract

Insights

Sperm mitochondrial dysfunction and altered proteins like NDUFS1, UQCRC2, and ATP1A4 may cause infertility in non-seminoma testicular cancer (NSTC) patients. These proteins could be biomarkers for NSTC-related subfertility.

Area of Science:

  • Reproductive biology
  • Oncology
  • Proteomics

Background:

  • Non-seminoma testicular cancer (NSTC) patients often experience subfertility or infertility.
  • The molecular mechanisms underlying NSTC-associated male infertility are not fully understood.

Purpose of the Study:

  • To compare the sperm proteome of NSTC patients who cryopreserved sperm before treatment with that of healthy fertile men.
  • To identify differentially expressed proteins (DEPs) in NSTC patients' sperm.

Main Methods:

  • Semen parameters (volume, motility, concentration) were assessed.
  • Sperm proteomic analysis was conducted using liquid chromatography-tandem mass spectrometry.
  • Bioinformatic tools were used to identify DEPs, with key proteins validated by Western blot.

Main Results:

  • 189 DEPs were identified between NSTC patients and controls.
  • Validation confirmed underexpression of mitochondrial proteins (NDUFS1, UQCRC2) and ATP1A4 in NSTC patients.
  • These proteins are crucial for sperm function and fertilization.

Conclusions:

  • Sperm mitochondrial dysfunction is a potential cause of reduced sperm quality in NSTC patients.
  • Identified DEPs may serve as biomarkers for NSTC-related subfertility/infertility.
  • Dysregulation of sperm molecular mechanisms contributes to reduced fertilizing ability in NSTC patients.
Keywords:
Cancer therapyMale fertilityNon-seminomaSperm qualityTesticular cancer

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