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Published on: May 24, 2022
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Assessment of the endodontic microbiota of abscessed primary teeth using microarray technology
Yeliz Guven1, Nilufer Ustun1, Sermin D Aksakal1
1Department of Pedodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
Summary
Microarray analysis revealed diverse oral bacteria in abscessed primary teeth, with Fusobacterium nucleatum being most common. Specific bacterial combinations may increase pathogenicity, but no link to clinical symptoms was found.
Area of Science:
- Microbiology
- Dental Research
- Infectious Diseases
Background:
- Limited knowledge exists regarding the microbial composition of abscessed primary teeth.
- Understanding these microbes is crucial for effective treatment strategies.
Purpose of the Study:
- To identify 10 specific oral bacterial species in abscessed primary tooth root canals using microarray technology.
- To investigate potential associations between bacterial presence and clinical findings in these infections.
Main Methods:
- Samples were collected from 20 abscessed primary molars.
- Microarray technology (ParoCheck®) was used for semi-quantitative bacterial identification.
- Clinical data included pain, mobility, percussion sensitivity, and swelling.
Main Results:
- All 10 tested bacterial species were detected.
- Fusobacterium nucleatum (100%), Parvimonas micra (65%), Prevotella intermedia (45%), and Treponema denticola (45%) were most prevalent.
- Significant positive correlations were found between F. nucleatum and P. intermedia/P. micra, and between T. denticola and other species. No correlation with clinical findings was observed.
Conclusions:
- Microarray technology successfully identified diverse bacteria in abscessed primary tooth root canals.
- Specific bacterial combinations may enhance pathogenicity through synergistic effects.
- Further research is needed to understand the clinical implications of these microbial profiles.
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