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Updated: Jan 31, 2026

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Published on: April 18, 2019
Embryonic or Induced Pluripotent Stem Cell-Derived Hepatocellular Systems for HCV Culture
Xianfang Wu1, Viet Loan Dao Thi2,3
1Laboratory of Virology and Infectious Diseases, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY, USA. xwu02@rockefeller.edu.
Abstract:
Human-induced pluripotent stem cell-derived hepatocyte-like cells (iHeps) constitute a powerful tool for modeling hepatotropic pathogen infections in cell culture. Meanwhile, CRISPR-Cas9 technology enables precise editing of stem cell genomes to generate patient-specific disease models and thus development of personalized experimental systems. Here we present a detailed stepwise protocol for the differentiation of stem cells to hepatocyte-like cells for HCV studies in cell culture. We also outline the use of an inducible iCRISPR platform for the rapid and efficient modification of host factors of interest to better understand their function during HCV infection.
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