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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Infection01:20

Infection

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When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
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Stages of Infection01:26

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Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
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Defense Mechanism Against Infection01:26

Defense Mechanism Against Infection

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Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
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Sexually Transmitted Infections01:26

Sexually Transmitted Infections

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Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
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A Cell Culture Model for Persistent HCV Infection.

Victoria Castro1, Ginés Ávila-Pérez1, Lidia Mingorance1

  • 1Department of Molecular and Cellular Biology, Centro Nacional De Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|December 30, 2018
PubMed
Summary

This study presents a cell culture model for persistent hepatitis C virus (HCV) infection, enabling detailed investigation of viral replication and host interactions. The model aids in developing new therapies for chronic HCV, a global health concern.

Keywords:
AntiviralCell cultureHepatitis CHost-virus interactionPersistent infection

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Area of Science:

  • Virology
  • Immunology
  • Hepatology

Background:

  • Chronic hepatitis C virus (HCV) infection leads to liver disease and cancer globally.
  • Persistent HCV infection results from immune evasion and intracellular survival strategies.
  • HCV evades immune clearance, contributing to its long-term presence and pathogenesis.

Purpose of the Study:

  • To describe a methodology for generating persistently HCV-infected cell cultures.
  • To establish a model for studying prolonged HCV infection and host-virus interactions.
  • To illustrate applications of the model in drug discovery and host-targeting therapies.

Main Methods:

  • Generation of persistently HCV-infected cell cultures.
  • Monitoring of viral load and progeny virus production.
  • Application of chemical compounds and host-targeting shRNAs.

Main Results:

  • The cell culture model recapitulates persistent HCV RNA replication and protein expression.
  • Infectious progeny virus assembly and secretion are observed in the model.
  • The model facilitates the study of host-virus interactions in chronic HCV infection.

Conclusions:

  • A robust cell culture model for persistent HCV infection has been established.
  • This model is valuable for studying viral replication, pathogenesis, and host-virus interactions.
  • The model serves as a platform for screening antiviral compounds and host-targeting strategies.