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Dynamic Cellular Proteome Remodeling during SARS-CoV-2 Infection. Identification of Plasma Protein Readouts.
Fátima Milhano Dos Santos1, Jorge Vindel-Alfageme1, Sergio Ciordia1
1Functional Proteomics Laboratory, National Center for Biotechnology (CNB-CSIC), Darwin 3, Madrid 28049, Spain.
Journal of Proteome Research
|November 27, 2024
Summary
Proteomics reveals how SARS-CoV-2 infection remodels lung cells, identifying potential biomarkers for COVID-19 prognosis. This research aids in developing new diagnostic and therapeutic strategies for the pandemic.
Area of Science:
- Biochemistry
- Molecular Biology
- Virology
Background:
- The COVID-19 pandemic necessitates understanding SARS-CoV-2 infection mechanisms.
- Proteomics offers insights into cellular responses and potential therapeutic targets.
Purpose of the Study:
- To investigate proteome remodeling in human lung alveolar cells (A549-ACE2) upon SARS-CoV-2 infection.
- To identify potential diagnostic and prognostic protein biomarkers for COVID-19.
Main Methods:
- Quantitative proteomics and phosphoproteomics using TMT labeling.
- Analysis of SARS-CoV-2 infected A549-ACE2 cells.
- Targeted parallel reaction monitoring (PRM) for protein validation.
- Machine learning for biomarker selection.
Main Results:
- Identified 6802 proteins and 6428 phospho-sites in infected cells.
- Revealed time-dependent regulation of cellular processes due to viral intervention.
- Selected 29 differential proteins from patient serum, with 14 showing prognostic value.
Conclusions:
- Proteomic analysis elucidates SARS-CoV-2's impact on lung cell machinery.
- Identified protein biomarkers hold promise for COVID-19 diagnosis and prognosis.
- Targeting identified proteins and kinases may offer new clinical management strategies.
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