Related Experiment Video
Updated: Jan 31, 2026

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
Published on: July 11, 2025
Using Plant Virus Based Nanorods to Modulate the Differentiation of Human Bone Marrow Stem Cells
Abstract:
Tobacco mosaic virus (TMV) is a protypical nanorod-shaped bioparticles that has been used as a building block to construct a variety of self-assembled nanomaterials for different biomedical applications, including drug delivery, in vivo imaging, tumor immunotherapy and tissue engineering. In this work, the roles of TMV and its mutant TMV-RGD1 nanoparticles on the differentiation of human bone marrow stem cells (hBMSCs), an important process in bone regeneration, were carefully investigated. We observed that cells cultured on the TMV-RGD1 nanorods coated substrate showed significantly higher levels of gene and protein expression of osteo-specific markers osteocalcin (OCN) and bone morphogenetic protein 2 (BMP2). Investigation of alkaline phosphatase (ALP) activity and calcium deposition further confirmed that the TMV-RGD1 substrate could promote the osteogenesis and induce the mineralization of hBMSCs. On the other hand, the adipogenesis was downregulated on TMV and TMV-RGD1 coated substrates. Taken together, this study demonstrates for the first time the potential of TMV-RGD1 in promoting osteogenic differentiation of hBMSCs which can lead to future applications in clinical bone engineering.
Related Concept Videos
Plant Cells and Tissues
Bone Marrow Sampling and Transplants
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
Plant Cell Wall
Adult Stem Cells
Meristems and Plant Growth
Plant Tissue Culture

