Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast flocculation: quantification.

M Stratford1, M H Keenan

  • 1AFRC Institute of Food Research, Norwich Laboratory, U.K.

Yeast (Chichester, England)
|June 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid identification of the genus Dekkera/Brettanomyces, the Dekkera subgroup and all individual species.

International journal of food microbiology·2014
Same author

Yeast identification: reassessment of assimilation tests as sole universal identifiers.

Letters in applied microbiology·2011
Same author

Mode of antimicrobial action of vanillin against Escherichia coli, Lactobacillus plantarum and Listeria innocua.

Journal of applied microbiology·2004
Same author

5,6-dimethylxanthenone-4-acetic acid (DMXAA), a novel antivascular agent: phase I clinical and pharmacokinetic study.

British journal of cancer·2003
Same author

Candida davenportii sp. nov., a potential soft-drinks spoilage yeast isolated from a wasp.

International journal of systematic and evolutionary microbiology·2002
Same author

Sorbic acid resistance: the inoculum effect.

Yeast (Chichester, England)·2000
Same journal

Comparative Analysis of Stress Adaptation in the Yeast Microbiome of Cactus.

Yeast (Chichester, England)·2026
Same journal

Enhanced Production of Recombinant Thermophilic Xylanase X11P in Ogataea polymorpha via In-Silico Signal Peptide Discovery and Fed-Batch Fermentation.

Yeast (Chichester, England)·2026
Same journal

Sugar Metabolisms Altered By Undissociated Forms of Organic Acids Based on the Emergence of [GAR<sup>+</sup>] Cells in Saccharomyces cerevisiae.

Yeast (Chichester, England)·2026
Same journal

Methods to Study Mitochondrial Metabolism and Homeostasis in Fission Yeast.

Yeast (Chichester, England)·2026
Same journal

Genetic Tools in the Nakaseomyces clade for Evolutionary Comparisons of Signal Transduction Pathways.

Yeast (Chichester, England)·2026
Same journal

rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences.

Yeast (Chichester, England)·2026
See all related articles

Yeast flocculation, a process requiring mechanical agitation, was quantitatively measured using orbital shaking. Results showed that agitation parameters and yeast strain significantly influence flocculation, impacting critical cell density.

Area of Science:

  • Biotechnology
  • Microbiology
  • Bioprocess Engineering

Background:

  • Yeast flocculation is an important characteristic in brewing and biotechnology.
  • Quantitative measurement of flocculation traditionally requires mechanical agitation.
  • Understanding the factors influencing flocculation is crucial for optimizing bioprocesses.

Purpose of the Study:

  • To establish a practical and meaningful method for quantitatively measuring yeast flocculation.
  • To investigate the influence of mechanical agitation on yeast flocculation parameters.
  • To determine the relationship between agitation, cell density, and flocculation characteristics.

Main Methods:

  • Assessed various mechanical agitation methods for quantitative yeast flocculation measurement.

Related Experiment Videos

  • Selected orbital shaking as the most practical and informative method.
  • Quantified flocculation using parameters such as minimum agitation threshold, initial rate, equilibrium extent, and particle size.

    • Main Results:

    • Orbital shaking provided practical and meaningful quantitative measurements of yeast flocculation.
    • All measured flocculation parameters (threshold, rate, extent, particle size) were found to be strain-dependent.
    • Critical cell density functions emerged when agitation was the limiting factor, irrespective of the agitation method.

    Conclusions:

    • Orbital shaking is a suitable method for quantitative yeast flocculation analysis.
    • Yeast strain and agitation intensity are key determinants of flocculation behavior.
    • The observed critical cell density phenomena under limited agitation are likely not linked to the strength of yeast cell bonds.