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Updated: Jan 31, 2026

Isolation of Primary Murine Brain Microvascular Endothelial Cells
Published on: November 14, 2014
The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers
Mathias Kaiser1,2, Malgorzata Burek3, Stefan Britz4
1Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, 13125 Berlin, Germany. Mathias.Kaiser@mdc-berlin.de.
Abstract:
Microvascular endothelial cells are an essential part of many biological barriers, such as the blood⁻brain barrier (BBB) and the endothelium of the arteries and veins. A reversible opening strategy to increase the permeability of drugs across the BBB could lead to improved therapies due to enhanced drug bioavailability. Vanilloids, such as capsaicin, are known to reversibly open tight junctions of epithelial and endothelial cells. In this study, we used several in vitro assays with the murine endothelial capillary brain cells (line cEND) as a BBB model to characterize the interaction between capsaicin and endothelial tight junctions.
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