Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering genes driving developmental stage progression in prostate cancer through spatial transcriptomics.

Genes & diseases·2026

Same author

BMAL1 regulates tubular epithelial-derived exosomal miR-27a-3p to inhibit macrophage-myofibroblast transition and alleviate ischemia/reperfusion-induced renal fibrosis.

Theranostics·2026

Same author

Post-marketing safety profile of bictegravir/emtricitabine/tenofovir alafenamide: a FAERS pharmacovigilance study with EudraVigilance validation and mechanistic investigation of pancreatitis.

Virology journal·2026

Same author

Conjugation in Euplotes songi, a phylogenetically basal species in the genus Euplotes: Nuclear events and new pheromone gene variants.

European journal of protistology·2026

Same author

Hi-C calibration by chemically induced chromosomal interactions.

EMBO reports·2026

Same author

Condensin accelerates long-range intra-chromosomal interactions.

Nature communications·2026

Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026

Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026

Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026

Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026

Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026

Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 31, 2026

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Published on: August 25, 2011

Using time-lapse fluorescence microscopy to study gene regulation.

Fan Zou¹, Lu Bai²

¹Department of Physics, The Pennsylvania State University, University Park, PA 16802, United States; Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, United States.

Methods (San Diego, Calif.)

|January 2, 2019

Summary

This summary is machine-generated.

Time-lapse fluorescence microscopy tracks gene expression dynamics in single cells. This powerful technique reveals insights into cellular processes like growth, differentiation, and responses to stimuli.

More Related Videos

Time-lapse Imaging of Mitosis After siRNA Transfection

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Published on: July 17, 2013

Related Experiment Videos

Last Updated: Jan 31, 2026

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Published on: August 25, 2011

Time-lapse Imaging of Mitosis After siRNA Transfection

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Published on: July 17, 2013

Area of Science:

Molecular Biology
Cell Biology
Biophysics

Background:

Gene regulation is fundamental to cellular processes.
Understanding gene expression dynamics requires high-resolution temporal and spatial data.
Traditional methods often lack the resolution to capture single-cell gene expression dynamics.

Purpose of the Study:

To review standard experimental procedures for time-lapse fluorescence microscopy.
To highlight new technical developments in the field.
To showcase the utility of this method for studying gene regulation.

Main Methods:

Time-lapse fluorescence microscopy of single cells.
Utilizing fluorescent signals to probe gene expression over time.
Integration with flow-cell devices for studying responses to external stimuli.

Main Results:

This method captures dynamics, noise, movement, memory, inheritance, and coordination of gene expression.
It provides unique insights into cell growth, development, and differentiation.
External stimuli effects on gene regulation can be measured.

Conclusions:

Time-lapse fluorescence microscopy is a powerful tool for studying gene regulation.
Its single-cell resolution and spatiotemporal capacity yield information difficult to obtain otherwise.
Advancements continue to expand its applications in cell biology research.